Microarray analysis of circular RNAs in HCT-8 cells infected with Cryptosporidium parvum

We read with great interest the article by Yin et al. (Parasit Vectors 14:238, 2021). The authors found that Cryptosporidium infection induced significantly aberrant expression of circular RNA profiles in HCT-8 cells, a finding which has far-reaching implications. However, due to the high number of false positives caused by multiple comparisons, statistical methods for microarray analysis should be carefully selected. Accurate analysis results will provide a convincing basis for subsequent experiments. In addition, we recommend several more appropriate methods in this article.

Automated summary

The study by Yin et al. on Cryptosporidium infection-induced aberrant expression of circular RNA profiles in HCT-8 cells is of great interest. The authors recommend careful selection of statistical methods to avoid false positives in microarray analysis, and provide suggestions for more appropriate methods in the article. Accurate analysis results are crucial for providing a convincing basis for subsequent experiments.