Incoherent superposition of polychromatic light enables single-shot nondiffracting light-sheet microscopy

We demonstrate single-shot nondiffracting light-sheet microscopy by the incoherent superposition of dispersed polychromatic light sources. We characterized our technique by generating a Bessel light-sheet with a supercontinuum light-source and a C-light-sheet using a diode laser, and demonstrated its applicability to fluorescence microscopy. We emphasize that our method is easily implementable and compatible with the requirements of high-resolution microscopy. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Automated summary

In this study, single-shot nondiffracting light-sheet microscopy was demonstrated through the incoherent superposition of dispersed polychromatic light sources. The technique was characterized by generating a Bessel light-sheet with a supercontinuum light-source and a C-light-sheet using a diode laser, showing its applicability in fluorescence microscopy. The method was emphasized to be easily implementable and compatible with the requirements of high-resolution microscopy.