Journal
OPTICS EXPRESS
Volume 29, Issue 13, Pages 20105-20120Publisher
Optica Publishing Group
DOI: 10.1364/OE.425632
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Funding
- National Institutes of Health [R01CA187427, R01CA250512]
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The design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device with solid-state detectors and automated gain control is reported. The device showed clear benefits compared to existing devices, including improved measurement accuracy, optimized signal-to-noise ratio, and increased imaging speed.
We report the design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device incorporating solid-state detectors and automated gain control. For every excitation pulse (similar to 1 mu J 600 ps), this device records complete fluorescence decay from multiple spectral channels simultaneously within microseconds, using a dedicated UV enhanced avalanche photodetector and analog to digital convert (2.5 GS/s) in each channel. Fast (<2 ms) channel-wise dynamic range adjustment maximizes the signal-to-noise ratio. Fluorophores with known lifetime ranging from 0.5-6.0 ns were used to demonstrate the device accuracy. Current results show the clear benefits of this device compared to existing devices employing microchannel-plate photomultiplier tubes. This is demonstrated by 5-fold reduction of lifetime measurement variability in identical conditions, independent gain adjustment in each spectral band, and 4-times faster imaging speed. The use of solid-state detectors will also facilitate future improved performance and miniaturization of the instrument. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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