Multispectral fluorescence lifetime imaging device with a silicon avalanche photodetector

We report the design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device incorporating solid-state detectors and automated gain control. For every excitation pulse (similar to 1 mu J 600 ps), this device records complete fluorescence decay from multiple spectral channels simultaneously within microseconds, using a dedicated UV enhanced avalanche photodetector and analog to digital convert (2.5 GS/s) in each channel. Fast (<2 ms) channel-wise dynamic range adjustment maximizes the signal-to-noise ratio. Fluorophores with known lifetime ranging from 0.5-6.0 ns were used to demonstrate the device accuracy. Current results show the clear benefits of this device compared to existing devices employing microchannel-plate photomultiplier tubes. This is demonstrated by 5-fold reduction of lifetime measurement variability in identical conditions, independent gain adjustment in each spectral band, and 4-times faster imaging speed. The use of solid-state detectors will also facilitate future improved performance and miniaturization of the instrument. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

Automated summary

The design, development, and characterization of a novel multi-spectral fluorescence lifetime measurement device with solid-state detectors and automated gain control is reported. The device showed clear benefits compared to existing devices, including improved measurement accuracy, optimized signal-to-noise ratio, and increased imaging speed.