Journal
ODONTOLOGY
Volume 109, Issue 4, Pages 895-903Publisher
SPRINGER
DOI: 10.1007/s10266-021-00620-5
Keywords
Dental pulp stromal cells; Decellularized dental pulp extracellular matrix; Bone morphogenetic proteins; BMP4; Dental pulp-like tissue regeneration
Categories
Funding
- National Natural Science Foundation of China [81301551]
- Natural Science Foundation of Chongqing, China [cstc2018jcyjAX0731, cstc2020jcyj-msxmX0380]
Ask authors/readers for more resources
The study investigates the potential of decellularized dental pulp extracellular matrix (dECM) combined with bone morphogenetic protein 4 (BMP4) in regulating dental pulp stromal cells (DPSC) for pulp regeneration. The results show that dECM promotes DPSC proliferation, and the combination of BMP4 and dECM accelerates the formation of pulp-like tissue by DPSCs in vitro.
Pulp regeneration with stem cells is a promising alternative for treating periapical and pulp diseases of young permanent teeth. The aim of this study was to characterize decellularized dental pulp extracellular matrix (dECM) and investigate whether bone morphogenetic protein 4 (BMP4) regulates dental pulp stromal cells (DPSC)-mediated pulp regeneration combined with dECM. Dental pulp isolated from healthy third molars was decellularized with 10% sodium dodecyl sulfate (SDS) and Triton X-100. H&E staining, DAPI staining and electron microscopy were used to observe the dECM structure. The Cell Counting Kit-8 assay was used to analyse cell proliferation. Recombinant adenovirus was used to overexpress BMP4 in DPSCs. The cells were cultured in dECM and dECM + three-dimensional (3D) Vitrogel systems, and bone/dentin/angiogenesis marker expression was evaluated by real-time polymerase chain reaction (RT-PCR) and ALP staining. DPSCs mixed with dECM and BMP4 were transplanted into nude mice, and pulp-like tissue formation was evaluated. The expression of osteogenic and angioblastic genes was increased, and pulp-like tissue formed in vivo. Thus, dECM promotes DPSC proliferation, BMP4 and dECM together accelerate pulp-like tissue formation by DPSCs in vitro.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available