Journal
NUCLEIC ACIDS RESEARCH
Volume 49, Issue 12, Pages 6660-6672Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab468
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Funding
- European Union's Horizon 2020 Research and Innovation programme under the Marie Sklodowska-Curie Actions [751931]
- National Institutes of Health [R01 GM121844]
- Marie Curie Actions (MSCA) [751931] Funding Source: Marie Curie Actions (MSCA)
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This study reveals the potential of B-CePs as probes for RNA structures and confirms their ability to help investigate RNA systems that are not readily amenable to traditional high-resolution techniques.
Elucidating the structure of RNA and RNA ensembles is essential to understand biological functions. In this work, we explored the previously uncharted reactivity of bis-chloropiperidines (B-CePs) towards RNA. We characterized at the molecular level the different adducts induced by the fast reacting compound B-CeP 1 with RNA. Following an approach based on solution thermal melting coupled with ESI mass spectrometry (STHEM-ESI), we proved the ability of B-CePs to induce inter-molecular cross-links between guanines in double stranded RNA. These results open the possibility of using B-CePs as structural probes for investigating higher-order structures, such as the kissing loop complex established by the dimerization initiation site (DIS) of the HIV-1 genome. We confirmed the potential of B-CePs to reveal the identity of RNA structures involved in long-range interactions, expecting to benefit the characterization of samples that are not readily amenable to traditional high-resolution techniques, and thus promoting the elucidation of pertinent RNA systems associated with old and new diseases. [GRAPHICS] .
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