Journal
NUCLEIC ACIDS RESEARCH
Volume 49, Issue W1, Pages W505-W509Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab427
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Funding
- Lundbeck Foundation [R230-2016-2986, R324-2019-1832]
- Aarhus University
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Prime editing is a precise genome editing technology that uses pegRNAs as guide molecules and editing templates. pegIT is a user-friendly web tool for rapid design of various user-defined edits.
Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecules and as templates carrying the desired edits. Due to such diverse functions, manual pegRNA design is a subject to error and not suited for large-scale setups. Here, we present pegIT, a user-friendly web tool for rapid pegRNA design for numerous user-defined edits, including large-scale setups.
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