Journal
FUNGAL BIOLOGY
Volume 120, Issue 2, Pages 125-136Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2015.04.007
Keywords
DNA barcoding; Fungal identification; Intraspecies/interspecies genetic diversity; ITS region; Pathogenic fungi; Reference ITS database
Categories
Funding
- NHMRC grant [APP1031952]
Ask authors/readers for more resources
Correct species identification is becoming increasingly important in clinical diagnostics. Till now, many mycological laboratories rely on conventional phenotypic identification. But this is slow and strongly operator-dependent. Therefore, to improve the quality of pathogen identification, rapid, reliable, and objective identification methods are essential. One of the most encouraging approaches is molecular barcoding using the internal transcribed spacer (ITS) of the rDNA, which is rapid, easily achievable, accurate, and applicable directly from clinical specimens. It relies on the comparison of a single ITS sequence with a curated reference database. The International Society for Human and Animal Mycology (ISHAM) working group for DNA barcoding has recently established such a database, focusing on the majority of human and animal pathogenic fungi (ISHAM-ITS, freely accessible at http://www.isham.org/ or directly from http://its.mycologylab.org). For some fungi the use of secondary barcodes may be necessary. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available