Journal
FUNGAL BIOLOGY
Volume 120, Issue 2, Pages 173-178Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2015.09.006
Keywords
Candida diagnostics; Chromogenic media; Malassezia; NCAC; Non-Candida albicans Candida
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Funding
- National Natural Science Foundation of China [31060006, 31260029]
- International Scientific and Technological Cooperation Program of Guizhou [7006]
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Rapid identification of pathogenic yeasts is a crucial step in timely and appropriate antifungal therapy. For diagnostics in the clinical laboratory, simplified alternatives to barcoding are needed. CandiSelect 4 (CS4) medium, a chromogenic medium for isolation of clinical yeasts, allows routine recognition of Candida albicans and presumptive identification of Candida tropicalis, Candida glabrata, and Candida krusei. We evaluated an extension of this method with 46 non-Candida albicans Candida (NCAC) and 7 Malassezia species. The medium supported growth of all species tested and a wide diversity of cultural types were observed. Colony colours were in violet, turquoise (including green and blue), or white tinges. Eight NCAC species produced violet pigmentation similar to that of C. albicans. Most NCAC species, including C. glabrata and C. tropicalis were distributed in the turquoise group. Malassezia species were invariably blue. (C) 2015 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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