Dynamical prefrontal population coding during defensive behaviours

Coping with threatening situations requires both identifying stimuli that predict danger and selecting adaptive behavioural responses to survive(1). The dorsomedial prefrontal cortex (dmPFC) is a critical structure that is involved in the regulation of threat-related behaviour(2-4). However, it is unclear how threat-predicting stimuli and defensive behaviours are associated within prefrontal networks to successfully drive adaptive responses. Here we used a combination of extracellular recordings, neuronal decoding approaches, pharmacological and optogenetic manipulations to show that, in mice, threat representations and the initiation of avoidance behaviour are dynamically encoded in the overall population activity of dmPFC neurons. Our data indicate that although dmPFC population activity at stimulus onset encodes sustained threat representations driven by the amygdala, it does not predict action outcome. By contrast, transient dmPFC population activity before the initiation of action reliably predicts avoided from non-avoided trials. Accordingly, optogenetic inhibition of prefrontal activity constrained the selection of adaptive defensive responses in a time-dependent manner. These results reveal that the adaptive selection of defensive responses relies on a dynamic process of information linking threats with defensive actions, unfolding within prefrontal networks.

Automated summary

The study found that threat representations and avoidance behavior initiation are dynamically encoded in the overall population activity of dmPFC neurons in mice. While dmPFC population activity at stimulus onset encodes sustained threat representations driven by the amygdala, it does not predict action outcome. Transient dmPFC population activity before the initiation of action reliably predicts avoided from non-avoided trials, revealing the dynamic process of information linking threats with defensive actions within prefrontal networks.