Biomineralization improves the stability of a Streptococcus pneumoniae protein vaccine at high temperatures

Aim: Protein vaccines have been the focus of research for vaccine development due to their safety record and facile production. Improving the stability of proteins is of great significance to the application of protein vaccines. Materials & methods: Based on the proteins pneumolysin and DnaJ of Streptococcus pneumoniae, biomineralization was carried out to prepare protein nanoparticles, and their thermal stability was tested both in vivo and in vitro. Results: Mineralized nanoparticles were formed successfully and these calcium phosphate-encapsulated proteins were resistant to proteinase K degradation and were thermally stable at high temperatures. The mineralized proteins retained the immunoreactivity of the original proteins. Conclusion: Mineralization technology is an effective means to stabilize protein vaccines, presenting a safe and economical method for vaccine administration.

Automated summary

This study focused on improving the stability of protein vaccines by biomineralization technology, successfully preparing mineralized protein nanoparticles that were resistant to degradation and thermally stable. The mineralized proteins retained their immunoreactivity, providing a safe and cost-effective method for vaccine administration.