Licochalcone A-loaded solid lipid nanoparticles improve antischistosomal activity in vitro and in vivo

Aim: To isolate licochalcone A (LicoA) from licorice, prepare LicoA-loaded solid lipid nanoparticles (L-SLNs) and evaluate the L-SLNs in vitro and in vivo against Schistosoma mansoni. Materials & methods: LicoA was obtained by chromatographic fractionation and encapsulated in SLNs by a modified high shear homogenization method. Results: L-SLNs showed high encapsulation efficiency, with satisfactory particle size, polydispersity index and Zeta potential. Transmission electron microscopy revealed that L-SLNs were rounded and homogenously distributed. Toxicity studies revealed that SLNs decreased the hemolytic and cytotoxic properties of LicoA. Treatment with L-SLNs showed in vivo efficacy against S. mansoni. Conclusion: L-SLNs are efficient in reducing worm burden and SLNs may be a promising delivery system for LicoA to treat S. mansoni infections.

Automated summary

This study successfully isolated LicoA from licorice, encapsulated it in L-SLNs, and demonstrated its effective in vivo efficacy against Schistosoma mansoni. The findings suggest that L-SLNs can effectively reduce worm burden and may serve as a promising delivery system for treating S. mansoni infections.