Unraveling the Photodynamic Activity of Cationic Benzoporphyrin-Based Photosensitizers against Bladder Cancer Cells

In this study, we report the preparation of new mono-charged benzoporphyrin complexes by reaction of the appropriate neutral benzoporphyrin with (2,2 '-bipyridine)dichloroplatinum(II) and of the analogs' derivatives synthesized through alkylation of the neutral scaffold with iodomethane. All derivatives were incorporated into polyvinylpyrrolidone (PVP) micelles. The ability of the resultant formulations to generate reactive oxygen species was evaluated, mainly the singlet oxygen formation. Then, the capability of the PVP formulations to act as photosensitizers against bladder cancer cells was assessed. Some of the studied formulations were the most active photosensitizers causing a decrease in HT-1376 cells' viability. This creates an avenue to further studies related to bladder cancer cells.

Automated summary

This study describes the preparation of new mono-charged benzoporphyrin complexes and their derivatives, which were incorporated into PVP micelles. The ability of these formulations to generate reactive oxygen species, specifically singlet oxygen, was evaluated, as well as their effectiveness as photosensitizers against bladder cancer cells. Some formulations showed promising results in reducing viability of HT-1376 cells, indicating potential for further studies related to bladder cancer cells.