Journal
MOLECULES
Volume 26, Issue 18, Pages -Publisher
MDPI
DOI: 10.3390/molecules26185572
Keywords
multiple-component optimization; Placket-Burman design; central composite design; macroporous resin; astragalus saponin
Funding
- Zhejiang Provincial Natural Science Foundation [LZ18H270001]
- National Science Foundation of China [81873226, 81630105, 81973560]
- Zhejiang Provincial Science and Technology Innovation Leading Talent Project of Ten Thousand Talents Plan (2019)
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This study optimized the extraction/purification process of multiple components using the entropy weight method combined with design methods, with Astragalus saponins as a case study. By screening significant factors through PBD and CCD, the optimal purification conditions were determined, leading to a successful practical application of the method to extract and purify natural products.
In this paper, the optimization of the extraction/purification process of multiple components was performed by the entropy weight method (EWM) combined with Plackett-Burman design (PBD) and central composite design (CCD). We took the macroporous resin purification of Astragalus saponins as an example to discuss the practicability of this method. Firstly, the weight of each component was given by EWM and the sum of the product between the componential content and its weight was defined as the comprehensive score, which was taken as the evaluation index. Then, the single factor method was adopted for determining the value range of each factor. PBD was applied for screening the significant factors. Important variables were further optimized by CCD to determine the optimal process parameters. After the combination of EWM, PBD and CCD, the resulting optimal purification conditions were as follows: pH value of 6.0, the extraction solvent concentration of 0.15 g/mL, and the ethanol volume fraction of 75%. Under the optimal conditions, the practical comprehensive score of recoveries of saponins was close to the predicted value (n = 3). Therefore, the present study provided a convenient and efficient method for extraction and purification optimization technology of multiple components from natural products.
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