Journal
MOLECULES
Volume 26, Issue 16, Pages -Publisher
MDPI
DOI: 10.3390/molecules26164746
Keywords
black garlic; 5-Hydroxymethylfurfural; 3-deoxyglucosone; epigallocatechin gallate; LC-MS; MS
Funding
- Ministry of Science and Technology, Republic of China [MOST 107-2628-B-005-002]
- Ajins Biomedical Co., Ltd. [AS-2019002-aa2]
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Impregnation of black garlic with epigallocatechin gallate (EGCG) was found to decrease the formation of the intermediate product 3-deoxyglucosone (3-DG) and further attenuate 5-HMF formation, possibly due to an additional reaction with 3-DG accelerated by EGCG. This study provides new insights regarding the role of EGCG in blocking 5-HMF formation.
5-Hydroxymethylfurfural (5-HMF) is a harmful substance generated during the processing of black garlic. Our previous research demonstrated that impregnation of black garlic with epigallocatechin gallate (EGCG) could reduce the formation of 5-HMF. However, there is still a lack of relevant research on the mechanism and structural identification of EGCG inhibiting the production of 5-HMF. In this study, an intermediate product of 5-HMF, 3-deoxyglucosone (3-DG), was found to be decreased in black garlic during the aging process, and impregnation with EGCG for 24 h further reduced the formation of 3-DG by approximately 60% in black garlic compared with that in the untreated control. The aging-mimicking reaction system of 3-DG + EGCG was employed to determine whether the reduction of 3-DG was the underlying mechanism of decreased 5-HMF formation in EGCG-treated black garlic. The results showed that EGCG accelerated the decrease of 3-DG and further attenuated 5-HMF formation, which may be caused by an additional reaction with 3-DG, as evidenced by LC-MS/MS analysis. In conclusion, this study provides new insights regarding the role of EGCG in blocking 5-HMF formation.
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