Strategies for Preparation of Chiral Stationary Phases: Progress on Coating and Immobilization Methods

Enantioselective chromatography is one of the most used techniques for the separation and purification of enantiomers. The most important issue for a specific successful enantioseparation is the selection of the suitable chiral stationary phase (CSP). Different synthetic approaches have been applied for the preparation of CSPs, which embrace coating and immobilization methods. In addition to the classical and broadly applied coating and immobilization procedures, innovating strategies have been introduced recently. In this review, an overview of different methods for the preparation of coated and immobilized CSPs is described. Updated examples of CSPs associated with the various strategies are presented. Considering that after the preparation of a CSP its characterization is fundamental, the methods used for the characterization of all the described CSPs are emphasized.

Automated summary

Enantioselective chromatography is a commonly used technique for separating and purifying enantiomers, with the key factor being the selection of a suitable chiral stationary phase (CSP). Various synthetic approaches, including coating and immobilization methods, have been used for preparing CSPs. In addition to traditional methods, innovative strategies have recently been introduced. This review provides an overview of methods for preparing coated and immobilized CSPs, along with emphasizing the importance of characterizing the CSPs after preparation.