RNA velocity-current challenges and future perspectives

RNA velocity has enabled the recovery of directed dynamic information from single-cell transcriptomics by connecting measurements to the underlying kinetics of gene expression. This approach has opened up new ways of studying cellular dynamics. Here, we review the current state of RNA velocity modeling approaches, discuss various examples illustrating limitations and potential pitfalls, and provide guidance on how the ensuing challenges may be addressed. We then outline future directions on how to generalize the concept of RNA velocity to a wider variety of biological systems and modalities.

Automated summary

RNA velocity has revolutionized the field of single-cell transcriptomics by providing directed dynamic information about gene expression kinetics. Researchers discuss current modeling approaches, limitations, potential pitfalls, and future directions for generalizing RNA velocity to diverse biological systems.