4.7 Article

Recombinant Bovine and Human Osteopontin Generated by Chlamydomonas reinhardtii Exhibit Bioactivities Similar to Bovine Milk Osteopontin When Assessed in Mouse Pups Fed Osteopontin-Deficient Milk

Journal

MOLECULAR NUTRITION & FOOD RESEARCH
Volume 65, Issue 16, Pages -

Publisher

WILEY
DOI: 10.1002/mnfr.202000644

Keywords

bioactivities; Chlamydomonas reinhardtii; milk osteopontin; recombinant osteopontin

Funding

  1. Triton Algae Innovations, San Diego, USA

Ask authors/readers for more resources

The study shows that recombinant bovine OPN and recombinant human OPN exhibit similar effects to bovine milk OPN and mouse milk OPN on stimulating infant development.
Scope Osteopontin (OPN), a highly phosphorylated and glycosylated protein, is present in most body fluids, including milk. OPN appears at a high concentration in human milk (130-180 mg L-1), but not bovine milk (approximate to 18 mg mL(-1)). It is previously shown that milk OPN is involved in various biological processes and therefore may be a valuable infant formula additive. Methods and Results In the present study, recombinant bovine OPN (rbOPN) and recombinant human OPN (rhOPN) are generated in a Chlamydomonas reinhardtii (C. reinhardtii) algal expression system. The rbOPN and rhOPN are phosphorylated but not glycosylated. To assess the bioactivities of rbOPN and rhOPN and compare their bioactivities to those of bovine milk OPN (bmOPN), wild-type (WT) mouse pups nursed by OPN knock-out (KO) dams are orally fed bmOPN, rbOPN, and rhOPN daily from postnatal days 1-21 (P1-21). Effects of these OPNs on development of the brain, intestine, and immune function are evaluated. The results show that rbOPN and rhOPN exhibit effects similar to those of bmOPN as well as mouse milk OPN on stimulating proliferation of the small intestine, increasing brain myelination and cognitive development, and enhancing development of immune function. Conclusion rbOPN and rhOPN are likely to provide beneficial bioactivities when added to infant diets.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available