The effects of plant growth substances on the oil content and fatty acid composition of Ricinus communis L.: an in vitro study

Background Ricinus communis L. (castor bean) is valued for its oil and the performance of oil is closely related to its fatty acid composition. Thus, producing oil in vitro with favored fatty acid profiles is a promising research area and may also offer industrial opportunities. Material and Method In line with this, the total amount of oil and the fatty acid composition of the samples, which were endosperm and calli obtained by treatment of various doses of plant growth regulators were determined. Results Results showed that the type and amount of the plant growth regulator used in the media affect the fatty acid composition. In detail, the biggest change was shown by Indole-3-Acetic Acid (IAA), in general, using the plant growth regulators at 5 mg L-1, instead of 20 mg L-1, was found to have induced larger differentiations. The effect of a natural plant growth regulator (IAA) on fatty acid profiles was bigger than the synthetic ones (NAA, 1-Naphthaleneacetic acid, and 2,4 D, 2,4-Dichlorophenoxyacetic acid). The media containing 5 mg L-1 of NAA, 20 mg L-1 of NAA, 20 mg L-1 of 2,4 D, or 5 mg L-1 of 2,4 D gave similar results.

Automated summary

This study examined the effects of different plant growth regulators on the fatty acid composition of Ricinus communis L. samples, showing that the type and concentration of plant growth regulators can significantly impact the fatty acid profiles, with IAA having the largest effect.