Journal
MOLECULAR AND CELLULAR PROBES
Volume 57, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2021.101730
Keywords
Getah virus; Swine; SYBR Green I real-Time quantitative reverse; transcription PCR
Categories
Funding
- National Natural Science Foundation of China [3177131377]
- Support plan for science and technology innovation team of colleges and universities in Henan Province [14IRTSTHN015]
Ask authors/readers for more resources
GETV, a mosquito-borne virus, is increasingly posing a threat to animal safety and public health. The newly developed RT-qPCR assay for GETV has high sensitivity and reproducibility, making it suitable for rapid diagnosis and quantitative analysis of GETV infection.
Getah virus (GETV), a mosquito-borne virus belonging to the Alphavirus genus of family Togaviridae, has become increasingly problematic, which poses a huge threat to the safety of animals and public health. In order to detect GETV quickly and accurately, we have developed a SYBR Green I real-time quantitative reverse transcription PCR (RT-qPCR) assay for GETV with the detection limit of 66 copies/mu L, excellent correlation coefficient (R-2) of 0.9975, and amplification efficiency (E) of 98.90%, the target selected was the non-structural protein 3 of GETV. The sensitivity of it was higher than that of ordinary RT-PCR by 1000 folds, and the inter-assay and intra-assay CV values were all less than 0.99%. The newly developed RT-qPCR assay exhibited good sensitivity and reproducibility, which will provide technical support for the reliable and specific rapid diagnosis, and quantitative analysis of GETV infection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available