Journal
MOLECULAR AND CELLULAR ENDOCRINOLOGY
Volume 530, Issue -, Pages -Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2021.111296
Keywords
RNAseq; Adrenal cortex; Zona glomerulosa; Zona fasciculata; Zona reticularis; Adrenal medulla
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Funding
- National Institutes of Health/National Institute of Diabetes and Digestive and Kidney Diseases [R01DK043140]
- American Heart Association [20CDA35320016]
- National Center for Advancing Translational SciencesMichigan Institute for Clinical and Health Research [UL1TR002240]
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This study demonstrates the application of targeted RNA sequencing to FFPE normal adrenal gland specimens, capturing distinct transcriptomic differences among adrenal zones. The data obtained will improve the understanding of transcriptomic patterns and potentially identify novel pathways controlling zone-specific steroid production.
Adequate access to fresh or frozen normal adrenal tissue has been a primary limitation to the enhanced characterization of the adrenal zones via RNA sequencing (RNAseq). Herein, we describe the application of targeted RNAseq to formalin-fixed paraffin-embedded (FFPE) normal adrenal gland specimens. Immunohistochemistry (IHC) was used to visualize and guide the capture of the adrenocortical zones and medulla. Following IHC-based tissue capture and isolation of RNA, high-throughput targeted RNAseq highlighted clear transcriptomic differences and identified differentially expressed genes among the adrenal zones. Our data demonstrate the ability to capture FFPE adrenal zone tissue for targeted transcriptomic analyses. Future comparison of normal adrenal zones will improve our understanding of transcriptomic patterns and help identify potential novel pathways controlling zone-specific steroid production.
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