Journal
MICROCHIMICA ACTA
Volume 188, Issue 7, Pages -Publisher
SPRINGER WIEN
DOI: 10.1007/s00604-021-04890-2
Keywords
Electroluminescence; Immunoassay; Faraday cage; Nanocomposite; Graphene oxide (GO); Ruthenium (II); Outer Helmholtz plane phi(2) (OHP); Quantum dots
Categories
Funding
- Biosensor Sciences and Technologies Research Center, Ardabil University of Medical Sciences, Ardabil, Iran [9522-95/11/5]
Ask authors/readers for more resources
A novel immunoassay based on co-reactant enhancing strategy was developed for the ECL determination of CA15-3 and CA72-4 tumor markers in real samples. The signaling and capture probes were carefully prepared and constructed, leading to accurate and reproducible detection results for the tumor markers.
A novel immunoassay is introduced based on co-reactant enhancing strategy for the electrochemiluminescent (ECL) determination of CA15-3 and CA72-4 tumor markers in real samples. For the preparation of the signaling probe, CA15-3 and CA72-4 antibodies first were labeled using Ru(bpy)(3)(2+)-N-hydroxysuccinimide ester (Ru(bpy)(3)(2+)-NHS) and conjugated with l-cysteine capped cadmium selenide (CdSe) quantum dots. Finally, it was cross-linked with chitosan-grafted graphene oxide (GO@CS) nanocomposite. The capture probe was constructed by deposition of multi-walled carbon nanotubes (MWCNT) at the surface of dual-working gold screen-printed electrodes (MWCNT-dwSPE) and covalent attachment of capture CA15-3 and CA72-4 antibodies to MWCNT-dwSPE. ECL signals were recorded by applying cyclic potential ranging from 0.3 to 1.1 V (vs. pseudo-reference Ag/AgCl) at the scan rate of 100 mV.s(-1). This immunoassay was used for determination of CA15-3 and CA72-4 in real samples the detection limits of 9.2 mu U.ml(-1) and 89 mu U.ml(-1) within linear ranges of 10 mu U.ml(-1)-500 U.ml(-1) and 100 mu U.ml(-1)-150 U.ml(-1), respectively. This immunoassay also showed acceptable accuracy with recoveries in the range 96.5-108 % and high reproducibility with RSD of 3.1 and 4.9.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available