4.7 Review

Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up

Journal

MICROBIAL CELL FACTORIES
Volume 20, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12934-021-01617-z

Keywords

Therapeutic proteins; Yeast; Secretion signal; Humanized yeast; Glycosylation

Ask authors/readers for more resources

Manufacture of recombinant therapeutics is a rapidly growing field in pharmaceuticals, with yeasts being established as important hosts for producing heterologous proteins. Advances in yeast gene manipulation tools and techniques have enabled customization of yeast cells for therapeutic protein synthesis, utilizing strategies such as secretory pathway engineering and glycosylation engineering.
The manufacture of recombinant therapeutics is a fastest-developing section of therapeutic pharmaceuticals and presently plays a significant role in disease management. Yeasts are established eukaryotic host for heterologous protein production and offer distinctive benefits in synthesising pharmaceutical recombinants. Yeasts are proficient of vigorous growth on inexpensive media, easy for gene manipulations, and are capable of adding post translational changes of eukaryotes. Saccharomyces cerevisiae is model yeast that has been applied as a main host for the manufacture of pharmaceuticals and is the major tool box for genetic studies; nevertheless, numerous other yeasts comprising Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Yarrowia lipolytica have attained huge attention as non-conventional partners intended for the industrial manufacture of heterologous proteins. Here we review the advances in yeast gene manipulation tools and techniques for heterologous pharmaceutical protein synthesis. Application of secretory pathway engineering, glycosylation engineering strategies and fermentation scale-up strategies in customizing yeast cells for the synthesis of therapeutic proteins has been meticulously described.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available