4.7 Editorial Material

Correlative approaches in single-molecule biophysics: A review of the progress in methods and applications

Related references

Note: Only part of the references are listed.
Article Biochemical Research Methods

TopoStats - A program for automated tracing of biomolecules from AFM images

Joseph G. Beton et al.

Summary: TopoStats is a Python toolkit that automates identification and tracing of individual molecules in Atomic Force Microscopy images without user input. It can accurately identify and trace a wide range of molecules within a field of view, including DNA minicircles, DNA origami rings, and pore forming proteins, with contour lengths typically within 10 nm of the predicted length. The program is freely available on GitHub and can be modified for different uses.

METHODS (2021)

Article Biochemical Research Methods

Single-molecule FRET dynamics of molecular motors in an ABEL trap

Maria Dienerowitz et al.

Summary: Single-molecule Fo center dot rster resonance energy transfer (smFRET) technique combined with the Anti-Brownian ELectrokinetic Trap (ABEL trap) allows for extended observation time of molecular motors without the need for tethered surface attachment, providing insight into their dynamics and conformational changes. The ABEL trap can selectively capture FRET active molecules and accelerate the data acquisition process, enabling high-resolution monitoring of molecular motors like Rep for multiple conformational switching events.

METHODS (2021)

Article Biochemical Research Methods

Integrating single-molecule spectroscopy and simulations for the study of intrinsically disordered proteins

Jhullian J. Alston et al.

Summary: Over the past two decades, intrinsically disordered proteins and protein regions have been recognized as essential drivers of cellular function, with singlemolecule fluorescence spectroscopy and molecular simulations providing insight into their behavior. When combined, these techniques offer complementary information that can help uncover complex molecular details.

METHODS (2021)

Article Biochemical Research Methods

Multi-dimensional and spatiotemporal correlative imaging at the plasma membrane of live cells to determine the continuum nano-to-micro scale lipid adaptation and collective motion

Miguel Bernabe-Rubio et al.

Summary: The assembly of the primary cilium in polarized epithelial cells involves the formation of the centrosome-associated membrane patch (CAMP) from the remnant-associated membrane patch (RAMP), from which the ciliary membrane stems. A correlative imaging approach has been developed to quantitatively analyze the lipid collective assembly at the plasma membrane over time and different spatial scales. These findings provide insights into the mechanisms behind the biogenesis of the ciliary membrane in polarized epithelial cells.

METHODS (2021)

Article Biochemistry & Molecular Biology

Single-molecule imaging of DNA gyrase activity in living Escherichia coli

Mathew Stracy et al.

NUCLEIC ACIDS RESEARCH (2019)

Article Biology

Molecular coordination of Staphylococcus aureus cell division

Victoria A. Lund et al.

ELIFE (2018)

Review Physics, Applied

The 2018 correlative microscopy techniques roadmap

Toshio Ando et al.

JOURNAL OF PHYSICS D-APPLIED PHYSICS (2018)

Review Cell Biology

Towards correlative super-resolution fluorescence and electron cryo-microscopy

Georg Wolff et al.

BIOLOGY OF THE CELL (2016)

Article Chemistry, Multidisciplinary

Super-Resolved Traction Force Microscopy (STFM)

Huw Colin-York et al.

NANO LETTERS (2016)

Article Chemistry, Physical

Analytical tools for single-molecule fluorescence imaging in cellulo

M. C. Leake

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2014)

Editorial Material Biology

The physics of life: one molecule at a time

Mark C. Leake

PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES (2013)

Review Biochemistry & Molecular Biology

Functioning Nanomachines Seen in Real-Time in Living Bacteria Using Single-Molecule and Super-Resolution Fluorescence Imaging

Sheng-Wen Chiu et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2011)

Article Chemistry, Multidisciplinary

Simultaneous Measurement of Ionic Current and Fluorescence from Single Protein Pores

Andrew J. Heron et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2009)

Article Chemistry, Multidisciplinary

Biocompatible force sensor with optical readout and dimensions of 6 nm3

H Shroff et al.

NANO LETTERS (2005)

Article Multidisciplinary Sciences

Simultaneous atomic force microscope and fluorescence measurements of protein unfolding using a calibrated evanescent wave

A Sarkar et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2004)

Article Engineering, Biomedical

Multiple sources of passive stress relaxation in muscle fibres

WA Linke et al.

PHYSICS IN MEDICINE AND BIOLOGY (2004)