Journal
MARINE DRUGS
Volume 19, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/md19060295
Keywords
epipolythiodioxopiperazines; sponge-derived fungus; Aspergillus quadrilineatus; Candida albicans; isocitrate lyase
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Funding
- Basic Science Research Program through the National Research Foundation of Korea - Ministry of Education, Science, and Technology [NRF-2018R1D1A1B07043375]
- National Research Foundation - Ministry of Science, ICT and Future Planning, Korea [NRF-2021R1A4A2001251]
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The study found that secoemestrin C (1), a fungal metabolite isolated from sponges, is a potent inhibitor of isocitrate lyase (ICL) in Candida albicans, affecting its growth by inhibiting mRNA expression under specific conditions.
Four epipolythiodioxopiperazine fungal metabolites (1-4) isolated from the sponge-derived Aspergillus quadrilineatus FJJ093 were evaluated for their capacity to inhibit isocitrate lyase (ICL) in the glyoxylate cycle of Candida albicans. The structures of these compounds were elucidated using spectroscopic techniques and comparisons with previously reported data. We found secoemestrin C (1) (an epitetrathiodioxopiperazine derivative) to be a potent ICL inhibitor, with an inhibitory concentration of 4.77 +/- 0.08 mu M. Phenotypic analyses of ICL-deletion mutants via growth assays with acetate as the sole carbon source demonstrated that secoemestrin C (1) inhibited C. albicans ICL. Semi-quantitative reverse-transcription polymerase chain reaction analyses indicated that secoemestrin C (1) inhibits ICL mRNA expression in C. albicans under C-2-assimilating conditions.
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