Journal
MARINE DRUGS
Volume 19, Issue 6, Pages -Publisher
MDPI
DOI: 10.3390/md19060320
Keywords
chitin oligosaccharides; chemo-enzymatic synthesis; lysozyme; response surface methodology
Categories
Funding
- Agence Nationale de la Recherche [NiceCrops ANR 14-CE18-0008]
- Bill and Melinda Gates Foundation (Engineering the nitrogen symbiosis for Africa, ENSA) [OPP1028264]
- Bill and Melinda Gates Foundation [OPP1028264] Funding Source: Bill and Melinda Gates Foundation
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Chitin oligosaccharides (COs) have high potential as organic fertilizers in agro-ecological transition, promoting symbiotic relationships between plants and microorganisms for nutrient uptake. Improved access to high-purity COs allows for further exploration of their complex roles in plants.
Chitin oligosaccharides (COs) hold high promise as organic fertilizers in the ongoing agro-ecological transition. Short- and long-chain COs can contribute to the establishment of symbiotic associations between plants and microorganisms, facilitating the uptake of soil nutrients by host plants. Long-chain COs trigger plant innate immunity. A fine investigation of these different signaling pathways requires improving the access to high-purity COs. Here, we used the response surface methodology to optimize the production of COs by enzymatic hydrolysis of water-soluble chitin (WSC) with hen egg-white lysozyme. The influence of WSC concentration, its acetylation degree, and the reaction time course were modelled using a Box-Behnken design. Under optimized conditions, water-soluble COs up to the nonasaccharide were formed in 51% yield and purified to homogeneity. This straightforward approach opens new avenues to determine the complex roles of COs in plants.
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