Tuning Dunaliella tertiolecta for Enhanced Antioxidant Production by Modification of Culture Conditions

Microalgae, a popular source of food and bioactive compounds, accumulate antioxidants in response to culture condition stresses. Using a factorial design (3 x 3), the effect of light, temperature, and nitrogen level on chlorophyll and carotenoids, total protein, total phenolic, ascorbate and glutathione content, and enzyme (catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD)) activities in Dunaliella tertiolecta was studied. Data were analysed using Design of Experiments (DoE), and recommendations are made for optimum cultivation conditions to achieve the highest antioxidant content (phenolics, ascorbate and glutathione) or enzyme (CAT, SOD, and POD) activities. This is the first study to apply three levels of three factors during cultivation to tune Dunaliella tertiolecta for optimal antioxidant production.

Automated summary

This study explored the impact of light, temperature, and nitrogen level on antioxidants and enzyme activities in microalgae, providing recommendations for optimal cultivation conditions. By applying a factorial design, it was shown that tuning microalgae cultivation conditions can lead to increased antioxidant content and enzyme activities.