Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 146, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2021.111470
Keywords
Bovine bone peptide; Double enzyme hydrolysis; Ultrasound; Molecular weight; Antioxidation
Categories
Funding
- Study and Demonstration on Utilization Technology of Edible By-products of Cattle and Sheep [2019-0202-NCC-0149]
- Industrialization of the Cascade Processing of Ecological Beef and Mutton [2020C-18]
- China Agriculture Research System [CARS-37]
- Key Technology Innovation and Industrial Application of High Quality Beef Cattle Quality Fattening and Green Processing [18YFINA075]
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An ultrasound-assisted double enzyme hydrolysis method was developed for extracting low molecular weight peptides from bovine bone. The study showed that the extracted peptides exhibited optimal antioxidant properties, making this method efficient for industrial extraction of bone peptides and resource re-utilization.
In this study, we developed an ultrasound-assisted double enzyme hydrolysis method for the extraction of low molecular weight peptides (LMWP) from bovine bone and determined the optimal process using response surface methodology (RSM). Moreover, we have discussed the effect of the extraction methods on the structure and antioxidant properties of bovine bone peptides (BBPs). Results from RSM studies showed that the optimal extraction yield of 21.04% was obtained when the experimental parameters were 76.25 x 104 U/g of the enzyme addition, an ultrasonic power of 413.90 W/L, pH of 9.00, and ultrasonic time of 2.01 h. The BBPs obtained using ultrasound-assisted enzyme hydrolysis (UDE) were appeared as small granules, uniform in structure, and exhibited a rough surface. Moreover, the BBP was found to have obvious amide I, II, and III band structures and an increase in hydrogen bonding, as determined using FTIR and NMR. In addition, the peptide extracted using UDE had the optimal antioxidant effect (P < 0.05). Thus, UDE is an efficient method for the extraction of LMWPs from bovine bone. This method can be used in an industrial extraction of bone peptides as it can have a positive effect on resource re-utilization of bovine bone.
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