Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 153, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2021.112481
Keywords
beta-galactosidase; Lactose; Lactiplantibacillus plantarum
Categories
Funding
- National Natural Science Foundation of China [31901665]
- Key Research and Development Program of Shan-dong Province [2020CXGC010602]
- Science and Technology Support Plan for Young People in Colleges and Universities of Shandong Prov-ince [2020KJE005]
- Young Doctor Cooperative Project of Qilu Univer-sity of Technology, Shandong Academy of Sciences [2019BSHZ0020]
- State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences [ZZ20200108]
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Transcriptome sequencing revealed the gene expression difference of Lactiplantibacillus plantarum in glucose and lactose medium, identifying that beta-galactosidase LacLM plays a major role in lactose utilization, providing a theoretical foundation for the growth and metabolism of Lp. plantarum in lactose medium.
Lactiplantibacillus plantarum has often been used for fermentation of lactose-rich substrates. However, its growth in lactose has not been studied at the molecular level and the role of different beta-galactosidases is also unknown. In this study, transcriptome sequencing was performed to reveal the difference of gene expression when Lp. plantarum LP01 was cultivated in glucose and lactose medium. Two genetic loci involved in lactose metabolism were highly transcripted by lactose induction, in which beta-galactosidase coding gene lacA, lacL and lacM were upregulated by 9.86, 9.12 and 9.53-fold, respectively. To explore the detail role of different beta-galactosidase, mutant strains Lp. plantarum Delta lacA, Lp. plantarum Delta lacLM and Lp. Plantarum Delta lacA Delta lacLM was constructed by gene knockout. Results showed that the growth of Lp. plantarum.lacA was slightly weakened in lactose medium, while the growth of Lp. plantarum Delta lacLM and Lp. plantarum Delta lacA Delta lacLM was completely inhibited. Furthermore, the growth of Lp. plantarum Delta lacLM could be restored after transformed into an complementation vector pNZ8148-PlacLM-lacLM. Therefore, the beta-galactosidase LacLM plays the major role in lactose utilization of Lp. plantarum, providing a theoretical foundation for the growth and metabolism of Lp. plantarum in lactose medium.
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