Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 144, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2021.111221
Keywords
Anthocyanin; Plant aqueous extracts; Microbiological safety; Milk keeping quality; Platform test
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This study introduced a new field-scale analytical tool for rapid screening of microbiological quality in raw milk using aqueous extracts of plant materials, specifically focusing on Hibiscus rosa-sinensis flower extract. The method showed high linearity, accuracy, and repeatability, making it a potential alternative for microbiological quality control in raw milk procurement.
In the present study, we propose a novel field-scale analytical tool for rapid screening of microbiological quality in raw milk using aqueous extracts of plant materials, i.e. flowers (e.g. Hibiscus rosa-sinensis L. and Clitoria ternatea), taproots (e.g. Beta vulgaris) and pricklypears (e.g. Opuntia dillenii). For each plant extract, the colour changes in raw milk were evaluated between 4.5 and 6.5 pH against the resazurin dye as a control. The plant anthocyanin content in each extract was analyzed by the differential pH method using a spectrophotometer. The Hibiscus rosa-sinensis flower extract was opted to further test since it had a pH-sensitive colour change (6.5; maroon to 6.2; light-pink) compared to other plant extracts, which did not indicate a noticeable colour variation with pH. Anthocyanin content of the Hibiscus extract was 0.59 g/mL. The novel method showed high linearity (R-2 = 0.95), 100% accuracy and greater repeatability with an intermediate level of precision. The limit of quantification and detection was 0.46 and 0.15 g/mL, respectively. In conclusion, we demonstrated the potential in using water extract of Hibiscus rosa-sinensis L. flowers as an alternative to the resazurin dye reduction method for rapid and accurate microbiological quality control for raw milk procurement in remote areas.
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