Comprehensive characterization reveals antimicrobial-resistant and potentially virulent Campylobacter isolates from poultry meat products in Southern Brazil

The aim of this study was to perform a comprehensive characterization of 48 Campylobacter isolates obtained from broiler meat products in Southern Brazil, which had been previously typed by pulsed-field gel electrophoresis (PFGE). The antimicrobial resistance (AMR) profile and the presence of 30 virulence-associated genes (VAGs) were investigated. Six AMR patterns and 26 VAG patterns were found, indicating a great genetic diversity among the isolates. It is noteworthy that 79.2% of Campylobacter isolates were resistant to one or more antimicrobial agents, and MDR was found in 35.4%, being more prevalent in C. jejuni isolates. Data analysis for the combination of AMR and VAG profiles indicated that the antimicrobial resistance and virulence profile were not related to a particular species. The combination of the PFGE, AMR and VAG sets of data improved the discrimination power. In addition, the presence of antimicrobial-resistant Campylobacter isolates carrying virulence genes such as hcp (T6SS marker), cgtB and wlaN (LOS biosynthesis markers), among others, represents a potential emerging threat to public health. The findings also demonstrate the need for more rigorous control measures for Campylobacter in the Brazilian poultry production chain to avoid the dissemination of antimicrobial resistant and virulent Campylobacter isolates through the poultry meat.

Automated summary

The study found high antimicrobial resistance and genetic diversity in Campylobacter isolates from broiler meat products in southern Brazil. Multidrug-resistant Campylobacter, especially in C. jejuni, was prevalent. Data analysis revealed that antimicrobial resistance and virulence profiles were not species-specific.