Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 147, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2021.111561
Keywords
Exopolysaccharide; Hypolipidemic activity; Lactobacillus rhamnosus; antioxidant activity
Categories
Funding
- Science and Technology Department of Zhejiang Province of China [2018C02048]
- Food Science and Engineering-the most important discipline of Zhejiang Province [2017SIAR216]
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Exopolysaccharides (EPS) produced by Lactobacillus rhamnosus ZFM231 isolated from milk were purified and separated into four fractions, showing antioxidant and hypolipidemic activities, with potential applications in the food and pharmaceutical industries.
Exopolysaccharides (EPS) produced by Lactobacillus rhamnosus ZFM231 isolated from milk were purified by DEAE Cellulose-52 and Sephadex G-100 chromatography in sequence, obtaining four purified fractions EPS-1, EPS-2, EPS-3 and EPS-4. Among them, EPS-1 was a neutral polysaccharide and the others were acidic polysaccharides. The four factions were characterized by chemical composition analysis, molecular weight measurement, and UV, IR, 1H and 13C NMR spectra analysis. The antioxidant activity assays indicated that the purified fractions possessed appreciable ferric iron reducing power, free radical (DPPH, hydroxyl and superoxide anion radicals) scavenging ability and ferrous iron chelating ability. These purified exopolysaccharides were also found to possess marked hypolipidemic activity in vitro. The results showed that they were able to effectively inhibit lipase activity, and possessed remarkable activity in reducing cholesterol and triglyceride levels in the HepG2 cell models. Therefore, Lactobacillus rhamnosus ZFM231 and EPS produced by this probiotic strain may find application in food and pharmaceutical industries.
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