Journal
LUPUS
Volume 30, Issue 9, Pages 1469-1480Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/09612033211020367
Keywords
Systemic lupus erythematosus; dendritic cells; proliferation
Categories
Funding
- Applied Basic Research Foundation of Yunnan Province, China [2017FE468(-004)]
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The highly expressed miR-564 promotes the maturation and proliferation of Mo-DC cells by negatively regulating TP53 expression. Transfection with miR-564 antagomir significantly inhibits the differentiation, maturation, and proliferation of Mo-DC cells. Co-transfection of miR-564 antagomir and TP53 si-RNA restores the proliferation and migration of DC cells in rescue experiments.
Background miRNA is involved in the occurrence and progression of systemic lupus erythematosus (SLE), but the regulatory effect of miRNA on dendritic cells in SLE patients is still unclear. Material and methods Bioinformatics methods were used to analyze the differentially expressed miRNA and its target genes in SLE patients. In vitro experiments were conducted to explore the effects and mechanisms of differentially expressed miRNAs in SLE patients on the differentiation and maturation of monocyte-derived dendritic cells. Results Bioinformatics analysis showed that miR-564 was up-regulated in SLE patients, and TP53 was the core target gene of miR-564. The expression level of miR-564 showed a rising trend during the differentiation and maturation of monocytes into Mo-DC cells. The differentiation, maturation and proliferation of Mo-DC cells were significantly inhibited by transfection with miR-564 antagomir. The expression of TP53 is negatively regulated by miR-564. In rescue experiments, the proliferation and migration of DC cells were significantly restored by co-transfection of miR-564 antagomir and TP53 si-RNA. Conclusion Highly expressed miR-564 promotes the maturation, proliferation of Mo-DC cells by negatively regulating the expression of TP53.
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