Journal
LABORATORY ANIMALS
Volume 56, Issue 3, Pages 270-278Publisher
SAGE PUBLICATIONS INC
DOI: 10.1177/00236772211045483
Keywords
Fibroblast-like cells; fish cell culture; fibroblasts; medaka; zebrafish; embryo; fin clip
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Funding
- Deutsche Forschungsgemeinschaft [FOR2509, TH1461/72, WI1824/9-1]
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The use of animal models allows for detailed studies on disease development and potential therapies, reducing the risk of therapeutic side effects for patients. Developing alternative test systems can help in reducing the number of experimental animals used in research, with cell culture systems showing promising potential.
In many human diseases, the molecular pathophysiological mechanisms are not understood, which makes the development and testing of new therapeutic approaches difficult. The generation and characterization of animal models such as mice, rats, fruit flies, worms or fish offers the possibility for in detail studies of a disease's development, its course and potential therapies in an organismal context, which considerably minimizes the risk of therapeutic side effects for patients. Nevertheless, due to the high numbers of experimental animals used in research worldwide, attempts to develop alternative test systems will help in reducing their count. In this regard, the cell culture system displays a suitable option due to its potential of delivering nearly unlimited material and the good opportunities for high-throughput studies such as drug testing. Here, we describe a quick and simple method to isolate and cultivate vital fibroblast-like cells from embryos and adults of two popular teleost model organisms, the Japanese rice fish medaka (Oryzias latipes) and the zebrafish (Danio rerio).
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