Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 94, Issue -, Pages 55-65Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2016.02.015
Keywords
Glutathione; Glutathione reductase; Mitochondria; Thioredoxin; Yeast
Funding
- Biotechnology and Biological Sciences Research Council (BBSRC) [BB/J00488X/1]
- BBSRC [BB/J00488X/1] Funding Source: UKRI
- MRC [MR/L011840/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/J00488X/1] Funding Source: researchfish
- Medical Research Council [MR/L011840/1] Funding Source: researchfish
Ask authors/readers for more resources
Glutathione is an abundant, low-molecular-weight tripeptide whose biological importance is dependent upon its redox-active free sulphydryl moiety. Its role as the main determinant of thiol-redox control has been challenged such that it has been proposed to play a crucial role in iron-sulphur clusters maturation, and only a minor role in thiol redox regulation, predominantly as a back-up system for the cytoplasmic thioredoxin system. Here, we have tested the importance of mitochondrial glutathione in thiol-redox regulation. Glutathione reductase (Girl) is an oxidoreductase which converts oxidized glutathione to its reduced form. Yeast Girl localizes to both the cytosol and mitochondria and we have used a Glr1(M1L) mutant that is constitutively localized to the cytosol to test the requirement for mitochondrial Glr1. We show that the loss of mitochondrial Glr1 specifically accounts for oxidant sensitivity of a girl mutant. Loss of mitochondrial Glr1 does not influence iron-sulphur cluster maturation and we have used targeted roGFP2 fluorescent probes to show that oxidant sensitivity is linked to an altered redox environment. Our data indicate mitochondrial glutathione is crucial for mitochondrial thiol-redox regulation, and the mitochondrial thioredoxin system provides a back-up system, but cannot bear the redox load of the mitochondria on its own. (C) 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available