Journal
JOURNAL OF TRANSLATIONAL MEDICINE
Volume 19, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s12967-021-02953-9
Keywords
Cervical cancer; hsa_circ_0000520; microRNA-1296; Cyclin-dependent kinase 2
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Funding
- Suzhou Citizen Life Science and Technology Plan Project [SYS201784]
- Suzhou High-tech Zone Medical and Health Science and Technology Plan Project [2016Q012, 2020Z008]
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The study found that hsa_circ_0000520 and CDK2 were highly expressed in cervical cancer tissues, and hsa_circ_0000520 could competitively bind to miR-1296 to affect CDK2 expression, thereby inhibiting cell proliferation and promoting apoptosis.
Background Circular RNA (circRNA) has been demonstrated to participate in cervical cancer development. In this study, we analyzed the role of hsa_circ_0000520 in cervical cancer. Methods Fifty-two pairs of cervical cancer and adjacent normal tissue samples were collected, and five human cervical cancer cell lines were obtained followed by the detection of hsa_circ_0000520 expression. Nuclear-cytoplasmic isolation and fluorescence in situ hybridization were performed to analyze the subcellular localization of hsa_circ_0000520 while linear RNA was digested by RNase R. Gain- or loss-of function experiments on hsa_circ_0000520 were performed, followed by detection of cell proliferation and cell cycle by EdU, Cell Counting Kit-8, colony formation assay, and flow cytometry respectively. Results Hsa_circ_0000520 and cyclin-dependent kinase 2 (CDK2) were highly expressed in cervical cancer tissues. Binding sites between microRNA-1296 (miR-1296) and hsa_circ_0000520 or CDK2 were verified. Antibody to Argonaute 2 (Ago2) could precipitate hsa_circ_0000520, indicating that hsa_circ_0000520 could competitively bind to miR-1296 via Ago2. Silencing hsa_circ_0000520 inhibited cervical cancer cell proliferation and promoted the inhibitory effects of miR-1296 on CDK2, thereby blocking cell cycle progression and promoting apoptosis. Conclusion These results support the premise that targeting hsa_circ_0000520 can be a potential approach to combat cervical cancer.
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