Journal
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Volume 102, Issue 1, Pages 280-290Publisher
WILEY
DOI: 10.1002/jsfa.11356
Keywords
fermented Dendrobium officinale polysaccharide; antioxidant activity; structure characterisation; anti-inflammatory activity
Funding
- National Key Research and Development Program of China [2017YFC1103800]
- Young Talents' Science and Technology Innovation Project of the Hainan Association for Science and Technology
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The polysaccharide extracted from the fermentation broth of Dendrobium officinale demonstrated strong antioxidant properties and protective effects against cytotoxicity induced by lipopolysaccharide. Additionally, it showed inhibitory effects on cytokines production.
BACKGROUND A polysaccharide was purified in this study, which was acquired from the fermentation broth of Dendrobium officinale Kimura et Migo. We aimed to investigate the structural features and bioactivity of this polysaccharide. RESULTS The polysaccharide was purified and the main polysaccharide fraction (i.e., DOP-1) was obtained. High-performance gel permeation chromatography (HPGPC) revealed that the molecular weight of DOP-1 was 447.48 kDa. Galactose, glucose and mannose were found to be present in DOP-1 via monosaccharide composition analysis, at a ratio of 1:1.79:6.71. Methylation and nuclear magnetic resonance spectroscopic analysis indicated that the backbone of DOP-1 was -> 4)-alpha-d-Glcp-(1 -> 4)-alpha-d-Manp-(1 -> 4)-alpha-d-Manp-(1 -> 4,6)-alpha-d-Manp-(1 ->, and its repeating units were also preliminarily established. In vitro tests proved that DOP-1 not only protects RAW264.7 macrophages from the cytotoxic effect induced by lipopolysaccharide (LPS), but also inhibits cytokines (i.e., interleukin-6 and tumour necrosis factor-alpha) induced by LPS. DOP-1 demonstrated good scavenging activity in vitro toward 1,1-diphenyl-2-picrylhydrazyl and hydroxyl radicals, as well as good metal chelating activity. Therefore, DOP-1 has potential antioxidant applications. CONCLUSION The structural characteristics of DOP-1 support its favourable biological activities and lay a strong foundation for further exploration of its structure-activity relationships and activity development, providing experimental data for the development and utilisation of fermentation broth of D. officinale. (c) 2021 Society of Chemical Industry.
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