Journal
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
Volume 168, Issue 6, Pages -Publisher
ELECTROCHEMICAL SOC INC
DOI: 10.1149/1945-7111/ac064d
Keywords
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Funding
- National Natural Science Foundation of China [21406093]
- Natural Science Foundation of Jiangsu Province [BK20140529]
- Open Project Program of State Key Laboratory of Food Science and Technology of Jiangnan University [SKLF-KF-201919]
- Key University Science Research Project of Jiangsu Province [14KJB530001]
- China Postdoctoral Science Foundation [2014M550271]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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This study developed a new biosensor based on modified glucose oxidase (mGOx) and carbon nanotubes for sensitive detection of glucose in honey. The biosensor showed a wide detection range, high sensitivity, low detection limit, good stability, reproducibility, and anti-interference properties. Importantly, it was successfully applied for real sample testing in the detection of glucose in honey.
In this study, a new biosensor based on modified glucose oxidase (mGOx) and carbon nanotubes was designed. Firstly, the glucose oxidase(GOx) was chemically modified by ionic liquid to obtain a new enzyme mGOx. Secondly, beta cyclodextrin (beta-CD) was combined with multi-wall carbon nanotubes (MWCNTs) to prepare beta-CD/MWCNTs composite carrier. Finally, mGOx was loaded on the beta-CD/MWCNTs composite material to construct a new biosensor mGOx/beta-CD/MWCNTs/GCE. And it was used for the determination of glucose in honey. The prepared enzyme biosensor could sensitively detect glucose, the detection range was between 50 mu M similar to 1.15 mM, the sensitivity was 35.44 mu A center dot mM(-1)cm(-2), and the detection limit was as low as 0.27 mu M. The biosensor had good stability (95.8% current retention within two weeks), reproducibility (n = 10, relative standard deviation (RSD) = 1.6%) and anti-interference. More importantly, actual samples test showed that the glucose biosensor could be successfully applied to the detection of glucose in honey.
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