Journal
JOURNAL OF THE AMERICAN NUTRITION ASSOCIATION
Volume 41, Issue 8, Pages 758-770Publisher
ROUTLEDGE JOURNALS, TAYLOR & FRANCIS LTD
DOI: 10.1080/07315724.2021.1961641
Keywords
Chrysin; adipogenesis; antioxidant; PPAR gamma
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Funding
- NPV Ramasamy Udayar PhD fellowship grant, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India
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The study demonstrates that chrysin effectively inhibits adipogenesis and lipogenesis while promoting lipolysis and antioxidant defense in 3T3-L1 adipocytes, showcasing its potential as an anti-adipogenic nutraceutical agent.
Objective: The present study was conducted to elucidate the in-vitro anti-oxidant and anti-adipogenic effect of the flavone, chrysin in comparison with the citrus bioflavonoid, hesperidin during adipogenic differentiation in 3T3-L1 mouse preadipocytes. Methods: The effect of chrysin and hesperidin on adipogenic differentiation was evaluated using Oil red-O staining, triglyceride estimation, free glycerol release, and ROS accumulation. The expression of adipogenesis-related genes was evaluated in real time-polymerase chain reaction. Results: 50 mu mol chrysin or hesperidin did not affect the cell viability of 3T3-L1 preadipocytes and adipocytes, but significantly reduced preadipocyte clonal population, accumulation of intracellular lipid and ROS and consequently increased lipolysis and antioxidant enzyme defence. It also decreased the expression of major adipogenic transcription factors, CCAAT/enhancer-binding protein-beta, peroxisome proliferator activated receptor-gamma, sterol regulatory element binding protein 1c, fatty acid synthase and hormone sensitive lipase. Conclusion(s): Herein we have indicated, for the first time, the effective anti-adipogenic mechanism of chrysin by down-regulating adipogenesis, lipogenesis and ROS and up-regulating lipolysis and antioxidant enzyme in differentiated 3T3-L1 adipocytes. As a nutritional bioflavonoid, chrysin with its more effective inhibition on adipogenesis than hesperidin has the potential to be developed as an anti-adipogenic nutraceutical agent.
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