Journal
JOURNAL OF RAMAN SPECTROSCOPY
Volume 52, Issue 8, Pages 1428-1439Publisher
WILEY
DOI: 10.1002/jrs.6177
Keywords
cell imaging; multivariate analysis; NWU-RSIT; Raman spectral imaging; univariate analysis
Categories
Funding
- National Natural Science Foundation of China [61911530695]
Ask authors/readers for more resources
An integrated Raman spectral imaging toolbox (NWU-RSIT) was developed for spectral analysis, image reconstruction, and feature recognition by combining univariate and multivariate algorithms. Different algorithms, such as PCA, HCA, KCA, VCA, and N-FINDR, were used to extract detailed composition information from hyperspectral dataset, making Raman spectral imaging more accessible for biomedical studies.
When consecutively acquiring Raman spectra at a given number, Raman imaging could be applied to extract the compositional and structural information from the selected sampling region. By compacting both univariate and multivariate algorithms, an integrated Raman spectral imaging toolbox (NWU-RSIT) was developed for spectral analysis, image reconstruction, and feature recognition. Using an example hyperspectral dataset from a single living mouse osteosarcoma cell, univariate imaging method reveals spatial distributions of some specific molecular vibration modes; multivariate algorithms, including principal component analysis (PCA), hierarchical clustering analysis (HCA), k-means clustering analysis (KCA), vertex component analysis (VCA), and N-FINDR algorithm, were realized to extract more detailed constitution information from the acquired hyperspectral dataset. After comparing and evaluating the system performance of NWU-RSIT, it will make Raman spectral imaging more accessible to all related fields, especially for the biomedical studies.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available