Preparation of a 99mTc-labeled graft polymer and its in vitro and in vivo evaluation

The aim of this study is the synthesis of a novel Tc-99m-labeld graft polymer and the biological evaluation of its in vitro and in vivo properties. To this end, a L-proline-graft-poly(HEMA) was prepared and labeled with Tc-99m. The radiochemical yield of approximately the Tc-99m-labeled compound amounted to 97 +/- 2.3%. The cytotoxicity test revealed no cytotoxic effect after a 24- and 48-h incubation. The results of the hemolysis test showed that hemolysis was non-toxic with an effect level of less than 2%. Subsequently, the biodistribution in healthy rats was determined. High accumulation of the polymer was observed in the pancreas, thyroid and prostate.

Automated summary

The study aimed to synthesize a novel Tc-99m-labeled graft polymer and evaluate its properties in vitro and in vivo. The labeled compound showed high radiochemical yield and non-toxic characteristics in cytotoxicity and hemolysis tests. Biodistribution studies in healthy rats demonstrated high accumulation of the polymer in the pancreas, thyroid, and prostate.