Journal
JOURNAL OF PROTEOMICS
Volume 244, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jprot.2021.104274
Keywords
Spirulina; Carbohydrate-active enzymes (CAZyme); Weaned piglet; Muscle proteome
Categories
Funding
- Fundacao para a Ciencia e a Tecnologia (FCT, Lisbon, Portugal) [PTDC/CVTNUT/5931/2014]
- Portugal 2020 [08/SI/3399/2015]
- CIISA [UIDB/00276/2020]
- LEAF [UIDB/04129/2020]
- FCT [SFRH/BD/143992/2019]
- FP7 VetMedZg Project of the European Commission [621394]
- Fundação para a Ciência e a Tecnologia [UIDB/00276/2020, UIDB/04129/2020, SFRH/BD/143992/2019, PTDC/CVT-NUT/5931/2014] Funding Source: FCT
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This study assessed the impact of Spirulina and lysozyme supplementation on piglet muscle proteome, revealing increased glycogen metabolism and nutrient reserves utilization in Spirulina group, and enhanced structural muscle protein synthesis with higher energy requirements in SP + L group. Proteomics offers valuable insight into the effects of dietary microalgae and enzyme activity on piglet metabolism.
Arthrospira platensis (Spirulina) is a microalga with a high content of crude protein. It has a recalcitrant cell wall that limits the accessibility of the animal endogenous enzymes to its intracellular nutrients. Enzymatic supplementation aiming to degrade cell walls could benefit microalgae digestibility. The objective of this study was to evaluate the impact of dietary Spirulina and lysozyme supplementation over the muscle proteome of piglets during the post-weaning stage. Thirty piglets were randomly distributed among three diets: control (no microalga), SP (10% Spirulina) and SP + L (10% Spirulina +0.01% lysozyme). After 4 weeks, they were sacrificed and samples of the longissimus lumborum muscle were taken. The muscle proteome was analysed using a Tandem Mass Tag (TMT)-based quantitative approach. A total of 832 proteins were identified. Three comparisons were computed: SP vs Ctrl, SP + L vs Ctrl and SP + L vs SP. They had ten, four and twelve differentially abundant proteins. Glycogen metabolism and nutrient reserves utilization are increased in the SP piglets. Structural muscle protein synthesis increased, causing higher energy requirements in SP + L piglets. Our results demonstrate the usefulness of proteomics to disclose the effect of dietary microalgae, whilst unveiling putative mechanisms derived from lysozyme supplementation. Data available via ProteomeXchange with identifier PXD024083. Significance: Spirulina, a microalga, is an alternative to conventional crops which could enhance the environmental sustainability of animal production. Due to its recalcitrant cell wall, its use requires additional measures to prevent anti-nutritional effects on the feeding of piglets in the post-weaning period, during which they endure post-weaning stress. One of such measures could be CAZyme supplementation to help degrade the cell wall during digestion. Muscle proteomics provides insightful data on the effect of dietary microalgae and enzyme activity on piglet metabolism.
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