Screening of sugar beet pre-breeding populations and breeding lines for resistance to Ditylenchus dipsaci penetration and reproduction

Ditylenchus dipsaci is an economically important plant-parasitic nematode affecting European sugar beets. To date, no sugar beet cultivars carrying resistance against D. dipsaci are available to farmers. To find potentially resistant sugar beet lines restricting reproduction and penetration of D. dipsaci, three consecutive in vivo bioassays were carried out. The first experiment determined the penetration rate of D. dipsaci in 79 breeding lines and 14 pre-breeding populations. Based on these results, D. dipsaci penetration and reproduction resistance of eight genotypes was intensively investigated. It could be demonstrated that none of the genotypes showed resistance towards D. dipsaci. However, a high variation of the penetration rate by D. dipsaci was observed among the genotypes. The breeding line 'DIT_119' effectively reduced D. dipsaci penetration (34.4 +/- 8.8 nematodes/plant at 22 days post-planting) compared to the susceptible control (109.0 +/- 16.9) while ensuring a yield comparable to non-inoculated plants. However, the breeding line 'DIT_119' did not reduce D. dipsaci reproduction. The paternal line of the cultivar BERETTA KWS, demonstrating a high tolerance to D. dipsaci crown rot symptoms, did not reduce penetration and reproduction. Thus, no correlation can be established between reduced penetration rates, reproduction, and tolerance to D. dipsaci. This study provides an essential basis for the development of resistant sugar beet cultivars to D. dipsaci. The variations observed among genotypes now need to be confirmed with larger-scale screenings.

Automated summary

This study conducted three consecutive in vivo bioassays to find potentially resistant sugar beet lines restricting reproduction and penetration of D. dipsaci. Although no resistance was found among the genotypes, a high variation of the penetration rate by D. dipsaci was observed. The study provides a basis for the development of resistant sugar beet cultivars to D. dipsaci, but further large-scale screenings are needed to confirm the observed variations among genotypes.