1H HR-MAS NMR-Based Metabolomic Fingerprinting to Distinguish Morphological Similarities and Metabolic Profiles of Maytenus ilicifolia, a Brazilian Medicinal Plant

Maytenus ilicifolia or Espinheira-Santa is a renowned Brazilian medicinal plant usually used against intestinal and stomach ulcers. Other species with similar thorny leaves have raised great confusion in order to discern the authentic M. ilicifolia. Misidentifications can lead to product adulteration of authentic M. ilicifolia with other species, which can be found on the Brazilian market. The intake of misclassified herbal products potentially could be fatal, demanding faster reliable fingerprinting-based classification methods. In this study, the use of H-1 HR-MAS NMR metabolomics fingerprinting and principal component analysis (PCA) allowed an evaluation of the authenticity for both collected and commercial M. ilicifolia samples, from the content of the flavanol, (-)-epicatechin (2), by observing variations in metabolic patterns. Plant specimen types from cultivated and natural habitats were analyzed by considering seasonal and topological differences. The interand intraplant topological metabolic profiles were found to be affected by seasonal and/or ecological trends such as sunlight, shade, rain, and the presence of pathogens. Moreover, several commercial samples, labeled as M. ilicifolia, were evaluated, but most of these products were of an inadequate quality.

Automated summary

The study evaluates the authenticity of collected and commercial M. ilicifolia samples using metabolomics fingerprinting and principal component analysis, showing that plant specimen types from different habitats and seasons may affect metabolic profiles.