Journal
JOURNAL OF MICROENCAPSULATION
Volume 38, Issue 7-8, Pages 472-485Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/02652048.2021.1979672
Keywords
Breast cancer; erlotinib; metformin; combined therapy; targeted drug delivery; magnetic nanoparticles
Funding
- Student Research Committee [58777]
- Research Center for Pharmaceutical Nanotechnology at Tabriz University of Medical Sciences
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This study aimed to develop therapeutic nanostructures encapsulating MET and ER for combinational therapy in breast cancer. The developed NSs showed a synergistic inhibitory impact on MCF-7 cells.
Aim This research aims to develop potential therapeutic nanostructures (NSs) encapsulating metformin (MET) and erlotinib (ER) for combinational therapy in breast cancer. Methods The ER and MET, both were loaded on mesoporous silica magnetic nanoparticles conjugated with polyethylene glycol and methotrexate to achieve targeted NSs. The developed NSs were characterised using SEM, DLS, and FTIR. Afterward, MTT, Trypan blue, and DNA extraction assays were operated for biological evaluations in the 2D and 3D MCF-7 cells. Results Physicochemical approaches indicated the mean diameter of 69.4 nm +/- 9.5 (PDI = 0.64), and neutral charge (2 mv) for the developed NSs. MET and ER-loaded NSs exhibited 62.56% +/- 4.41 and 67.73% +/- 3.03 drug release amount in pH = 5.4, respectively. MTT assay revealed that ER- and MET-loaded NSs had less metabolic activity (approximate to 20%) in comparison with non-targeted NSs. Conclusion Overall, our combined ER and MET-loaded targeted NSs result in a synergistic inhibitory impact on MCF-7 cells.
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