Journal
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
Volume 31, Issue 7, Pages 1022-1027Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
DOI: 10.4014/jmb.2105.05003
Keywords
Monoamine oxidases; chromenone derivatives; Streptomyces sp; CNQ-031; reversible competitive inhibitors
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Funding
- National Research Foundation of Korea (NRF) [NRF2019R1A2C1088967]
- Korean government of the Republic of Korea
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Three compounds isolated from marine-derived Streptomyces sp. CNQ-031 were evaluated for their inhibitory activities against neuronal enzymes, with Compound 1 and 2 showing potent inhibitory effects on MAO-A and MAO-B, respectively.
Three compounds were isolated from marine-derived Streptomyces sp. CNQ-031, and their inhibitory activities against monoamine oxidases (MAOs), acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and beta-secretase (BACE-1) were evaluated. Compound 1 (5,7-dihydroxy-2-isopropyl-4H-chromen-4-one) was a potent and selective inhibitor of MAO-A, with a 50% inhibitory concentration (IC50) of 2.70 mu M and a selectivity index (SI) of 10.0 versus MAO-B. Compound 2 [5,7-dihydroxy-2-(1-methylpropyl)-4H-chromen-4-one] was a potent and low-selective inhibitor of MAO-B, with an IC50 of 3.42 mu M and an SI value of 2.02 versus MAO-A. Compound 3 (1-methoxyphenazine) did not inhibit MAO-A or MAO-B. All three compounds showed little inhibitory activity against AChE, BChE, and BACE-1. The K-i value of compound 1 for MAO-A was 0.94 +/- 0.28 mu M, and the K-i values of compound 2 for MAO-A and MAO-B were 3.57 +/- 0.60 and 1.89 +/- 0.014 mu M, respectively, with competitive inhibition. The 1-methylpropyl group in compound 2 increased the MAO-B inhibitory activity compared with the isopropyl group in compound 1. Inhibition of MAO-A and MAO-B by compounds 1 and 2 was recovered by dialysis experiments. These results suggest that compounds 1 and 2 are reversible, competitive inhibitors of MAOs and can be considered potential therapies for neurological disorders such as depression and Alzheimer's disease.
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