Journal
FOOD SCIENCE AND TECHNOLOGY RESEARCH
Volume 22, Issue 5, Pages 673-678Publisher
KARGER
DOI: 10.3136/fstr.22.673
Keywords
tofu-misozuke; lactic acid bacteria; biogenic amines; tyrosine decarboxylase
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Tofu-misozuke is a traditional fermented food in Japan, and contains biogenic amines that function as neurotransmitters, but are toxic when consumed at high levels. Analysis of the amine production of bacterial communities is important for controlling the amine content in fermented foods. We investigated the bacterial community and biogenic amine content of tofu-misozuke. The lactic acid bacteria (LAB) ratio in the community and tyramine content were highly related. LAB were isolated to identify the biogenic amine-producing species. Isolated strains from tofu-misozuke were identified, and their tyramine formation potential was evaluated by PCR detection of the tyrosine decarboxylase gene and by cultivation tests. We detected three tyramine-producing bacteria, Enterococcus faecium, Weissella viridescens, and Lactobacillus curvatus, in tofu-misozuke.
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