Journal
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
Volume 32, Issue 9, Pages -Publisher
SPRINGER
DOI: 10.1007/s10856-021-06587-7
Keywords
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Funding
- National Natural Science Foundation of China [81672130]
- Military Medical Project [AWS14C007]
- Military Medical and Health Achievements Expansion Project [19WKS12]
- Natural Science Foundation of Liaoning Province [2019-BS-079]
- Liaoning Revitalization Talents Program [XLYC1807131]
- Science and Technology Innovation Foundation of Dalian [2020JJ27SN070]
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The study demonstrated that the combination of TBC with rhBMP-2 and Sr had good bioactivity, significant bone conduction, and excellent bone defect repair performance, potentially paving the way for clinical applications.
Objective: To study the bone induction and defect repair of true bone ceramics (TBC) combined with rhBMP-2 and Sr. Methods: MC3T3-E1 cells were used to evaluate the bioactivity of the composite. Cell proliferation activity was detected by CCK-8, ALP activity was detected by p-nitrophenyl phosphate (PNPP), and the differences of material surface topography were observed by scanning electron microscopy (SEM). Bone induction was verified by the implantation in nude mice. The rabbit femoral condyle defect model was achieved to verify the bone defect repair ability of the material. Results: SEM results showed nearly the same surface morphology and cell proliferation quantified by CCK-8 showed that compared with TBC, both TBC&Sr and TBC&BMP-2&Sr had a significant promoting effect (P < 0.05). ALP activity result showed that the ALP activity of TBC&BMP-2&Sr was significantly higher than that of TBC alone (P < 0.05). The bone induction result showed that TBC&Sr had a small amount of new bone formation, and the new bone area was only 2.5 +/- 0.11%. The bone induction activity of TBC&BMP-2&Sr was the highest, the new bone area was up to 75.36 +/- 4.21%. Histological result of bone defect repair showed that TBC&BMP-2&Sr was also the highest, the new bone area was up to 72.42 +/- 3.14%. The repair effect of TBC& BMP-2 was second, and better than that of TBC&Sr. Conclusion: TBC combined with rhBMP-2 and Sr had the good bioactivity, obvious bone conduction and bone defect repair performance, laying the foundation of clinical application potentially.
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