Biocompatibility studies of polyurethane electrospun membranes based on arginine as chain extender

Electrospun polymers are an example of multi-functional biomaterials that improve the material-cellular interaction and aimed at enhancing wound healing. The main objective of this work is to fabricate electrospun polyurethane membranes using arginine as chain extender (PUUR) in order to test the fibroblasts affinity and adhesion on the material and the polymer toxicity. Polyurethane membranes were prepared in two steps: (i) the polyurethane synthesis, and ii) the electrospinning process. The membranes were characterized by scanning electron microscopy (SEM), Fourier transforms infrared spectroscopy, gel permeation chromatography, and differential scanning calorimetry techniques. The evaluation of PUUR as a scaffolding biomaterial for growing and developing of cells on the material was realized by LIVE/DEAD staining. The results show that the fluorescent surface area of human fibroblasts (hFB), was greater in control dense membranes made from Tecoflex than in electrospun and dense PUUR. From SEM analysis, the electrospun membranes show relatively uniform attachment of cells with a well-spread shape, while Tecoflex dense membranes show a non-proliferating round shape, which is attributed to the fiber's structure in electrospun membranes. The cell morphology and the cell attachment assay results reveal the well spreading of hFB cells on the surface of electrospun PUUR membranes which indicates a good response related to cell adhesion. [GRAPHICS]

Automated summary

Electrospun polyurethane membranes were fabricated using arginine as chain extender to test fibroblasts affinity and adhesion, with results indicating greater fluorescent surface area on dense control membranes made from Tecoflex compared to electrospun and dense PUUR. SEM analysis revealed the well-spread shape of cells on electrospun membranes, attributing to the fiber's structure. Cell morphology and attachment assay results showed a good response related to cell adhesion on the surface of electrospun PUUR membranes.