Journal
FOOD RESEARCH INTERNATIONAL
Volume 87, Issue -, Pages 60-67Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2016.06.019
Keywords
Mimosa scabrella Bentham; Honeydew honey; Phenolic compounds; Dilute-and-shoot; LC-ESI-MS/MS; Method validation
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A simple, reproducible and sensitive method has been optimized and validated for simultaneous determination of 32 phenolic compounds in bracatinga (Mimosa scabrella Benth.) with the diluted-and-shoot approach, without the need of any additional clean-up steps. It has been based on high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry and electrospray ionization (HPLC-ESI-MS/MS). The chromatography conditions were optimized, and due to the selectivity provided by MRM monitoring, LC separation required only 9 min. The developed method was validated on the basis of Eurachem and European Commission Decision 2002/657/EC guidelines. Mean recoveries ranged from 70.4 to 110%. Intra-day and inter-day precision with RSD (relative standard deviations) from 0.14 to 18.9% and 034 to 20.0%, respectively were achieved. Limits of detection (LOD) and quantification (LOQ) ranged from 0.03 to 3.20 mu g L-1 and 0.20-12.8 mu g L-1. Finally, the method was applied to samples and 20 phenolic compounds were quantified in all the samples analyzed, representing a contribution to the characterization and quantification of phenolic compounds from bracatinga (M. scabrella Bentham) honeydew honey. (C) 2016 Elsevier Ltd. All rights reserved.
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