Journal
JOURNAL OF HAZARDOUS MATERIALS
Volume 416, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jhazmat.2021.126203
Keywords
TBBPA; Denitrification; Cu; Transmembrane transport; Electron transport
Categories
Funding
- National Natural Science Foundation of China [41701577, 42077361]
- Key Research and Development Projects of Anhui Province [202004i07020001]
- Natural Science Foundation of Anhui Province [1808085QD107]
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This study investigated the combined effect of TBBPA and Cu on model denitrifying bacteria, finding that Cu intensified the toxicity of TBBPA to denitrification by stimulating EPS secretion, blocking glucose transport, suppressing key enzyme activity, and down-regulating gene expression related to electron transport. These effects led to decreased bacterial growth, insufficient electron donor, and lower electron transport activity, causing a synergistic toxic effect of TBBPA and Cu on denitrification. Overall, this study provides new insights into the combined impact of BFRs and HMs on microorganisms.
Increasing electrical and electronic waste have raised concerns about the potential toxicity of brominated flame retardants (BFRs) and heavy metals (HMs). However, few studies have focused on the combined effect of BFRs and HMs on microorganisms, especially denitrifying bacteria, which have an essential role in N cycles and N2O emission. Herein, we investigate the combined effect of tetrabromobisphenol A (TBBPA) and Cu on model denitrifying bacteria. A further 24.5% decline in N removal efficiency was observed when 0.05 mg/L Cu were added into a denitrifying system containing 0.75 mg/L TBBPA. Further study demonstrated that Cu heightened the toxicity of TBBPA to denitrification via following aspects: (1) Cu stimulated EPS secretion induced by TBBPA during denitrification, blocked the transmembrane transport of glucose, which caused insufficient carbon substrate for bacteria growth and electron provision; (2) Cu further suppressed key denitrifying enzymes' activity and down-regulated genes involving electron transport induced by TBBPA, led to the decrease of electron transport activity. Finally, the decrease of bacterial growth, insufficient electron donor, and lower electron transport activity caused the synergetic toxic effect of TBBPA and Cu on denitrification. Overall, the present study provides new insights into the combined effect of BFRs and HMs on microorganisms.
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